Composite

Part:BBa_K2365046

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-14)


TEF1 promoter-CYC1 terminator

Between the TEF1 and cyc1,having the restriction enzyme cutting site.And you can insert the gene if you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 25
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 547


TEF1 TEF1-cyc1.jpeg

We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm

U-disk test.jpg
酵母荧光.jpg
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